Microbiology: Laboratory Theory and Application

Microbiology: Laboratory Theory and Application

Language: English

Pages: 888

ISBN: 1617312509

Format: PDF / Kindle (mobi) / ePub


Beautiful full-color photography, effective illustrations, carefully written exercises, and a reasonable price combine to make Microbiology: Laboratory Theory & Application the best-selling microbiology lab manual series on the market. This edition satisfies the content needs of courses populated by majors or in nonmajors courses where an increased coverage of applied microbiology is desired.

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mL portions into test tubes and cap loosely (Figure 1-4). As with agar slants, if your tubes are smaller than those recommended in Materials, add enough broth to fill them approximately 20% to 25%. 4 Sterilize the medium by autoclaving for 15 minutes at 121°C (Figure 1-5). Suspend the ingredients in one liter of distilled or deionized water in the two-liter flask. Agitate and heat slightly (if necessary) to dissolve them completely (Figure 1-3). Day Two 1 2 Examine the tubes and plates for

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152 EXERCISE 5-2 Oxidation–Fermentation Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 Fermentation Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158 EXERCISE 5-3 Phenol Red Broth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158 EXERCISE 5-4 Methyl Red and Voges-Proskauer Tests . . . . . . . . . . . . . . . . . . . 161

available) ࡗ Kinyoun carbolfuchsin ࡗ acid alcohol (95% ethanol ‫ ם‬3% HCl) ࡗ brilliant green stain ✦ sheep serum ✦ squirt bottle with water ✦ heating apparatus (steam or hotplate)—for ZN only ✦ nonsterile Petri dish for transporting slides ✦ disposable gloves ✦ lab coat ✦ eye goggles for ZN only ✦ recommended organisms: ࡗ Mycobacterium phlei (BSL-2) ࡗ Staphylococcus epidermidis Procedure: Ziehl-Neelsen (ZN) Method 1 Prepare a smear of each organism on a clean glass slide as illustrated in

fermentation. Figure A-5 on page 434 illustrates several reactions where carbon compounds are split and CO2 is released into the medium. Alcohols produced by fermentation are liquid and colorless, so they are not seen with the naked eye, and because they do not ionize in water they have minimal effect if any on the pH of the medium. The weak acids (e.g. acetic acid, propionic acid, formic acid, succinic acid, and butyric acid) have a dramatic effect on the pH of any medium and can easily be

required ✦ Agents associated with human disease ✦ Routes of transmission through percutaneous injury, ingestion, mucous membrane exposure BSL-1 practice plus: ✦ Limited access ✦ Biohazard warning signs ✦ “Sharps” precautions ✦ Biosafety manual defining any needed waste decontamination or medical surveillance policies Primary barriers: ✦ Class I or II BSCs or other physical containment devices used for all manipulations of agents that cause splashes or aerosols of infectious materials Personal

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