Cancer Prevention: Dietary Factors and Pharmacology (Methods in Pharmacology and Toxicology)

Cancer Prevention: Dietary Factors and Pharmacology (Methods in Pharmacology and Toxicology)

Language: English

Pages: 288

ISBN: 1493954466

Format: PDF / Kindle (mobi) / ePub

Focused on the discovery of precise molecular targets for the development of the cancer preventive agents, Cancer Prevention: Dietary Factors and Pharmacology provides researchers and non-researchers with practical methodologies for developing and validating small molecule and phytochemical-derived drug discovery and mechanisms by which these compounds can modulate distinct target proteins involved in oncogenic signaling. While this volume is primarily focused toward cancer prevention research, the range of techniques demonstrated in the book also provides an introduction of cancer prevention research methods to researchers outside the field. Chapters deal with a critical discussion of both laboratory and clinical topics, with each chapter containing both a discursive section along with a detailed methods section. As part of the Methods in Pharmacology and Toxicology series, this meticulous volume includes the kind of key implementation advice that seeks to ensure successful results in the lab.

Practical and authoritative, Cancer Prevention: Dietary Factors and Pharmacology aims to guide research toward identifying molecular targets and conducting human studies with phytochemicals which would, ideally, provide an enhanced approach to the goal of personalized cancer prevention.

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Molecular docking has become a standard tool in computational biology and, in combination with virtual screening, is used to envisage the binding orientation of target proteins with smallmolecule drug candidates in order to assess the affinity and activity of the small molecule. Thus, molecular docking plays an important role in the rational design of drugs. The GLIDE module from the Schrödinger Suite [21] provides a range of speed versus accuracy options and a variety of docking protocols. GLIDE

7. Incubate the tissue sections with a biotin-conjugated speciesspecific secondary antibody for 45 min at room temperature. 8. Incubate the slides with Streptavidin-HRP conjugate for 45 min at room temperature. 9. Rinse the slides 3 times in PBS-B for 5 min each. 10. Immediately before use, prepare the DAB substrate solution by adding 2 drops of Buffer Stock Solution, 4 drops of DAB Stock Solution and 2 drops of the Hydrogen Peroxide Solution to 5.0 mL of distilled water, and mixing well after

facility, F344 male rats (5–7 weeks old) are given s.c. injections of NMBA (0.25–0.35 mg/kg B.W.) three times per week for 5 weeks. One week after cessation of NMBA treatment, the esophagus contains a mixture of normal, hyperplastic, and dysplastic (mild to moderate) epithelium resembling the histopathology of esophagi in high-risk Chinese. At that point, the rats are fed 2.5, 5.0, or 10 % BRB diets until they are sacrificed at the end of the bioassay (30–35 weeks). The control groups are the

(NSCLC), which is usually divided into adenocarcinoma, squamous cell carcinoma (SCC), and large cell carcinoma. In order to illustrate the power of the mouse model in preclinical lung cancer investigations, comprehensive instructions for the selection of mice, genotyping, and induction of lung tumors (e.g., adenoma/adenocarcinoma, lung SCC, and SCLC) in mice are provided. We have described in detail the histological features of these tumors and the application of these features in lung cancer

Nottingham, UK), and 4 mL of glacial acetic acid. 8. 3 % H2O2: 30 mL of H2O2 (Sigma) and 970 mL of DDW. 9. 10 mM sodium citrate buffer: 2.94 g of sodium citrate in 800 mL of DDW. Adjust the pH to 6.0 and add DDW to 1 L. 10. 1 % DAB: 0.1 g of 3,3′-diaminobenzidine (DAB; Sigma) in 10 mL of DDW. Add 10N HCl three to five drops and solution turns light brown color. Shake for 10 min and DAB should dissolve completely. Aliquot and store at −20 °C. 11. 1× Cell lysis buffer: 20 mM Tris–HCl (Amresco LLC,

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